NASC

NASC ID: N849230

Name: WiscDsLox233237_21C

ABRC stock number: CS849230

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox233237_21C.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G76135 and AT1G76140.1-P355 (chr 1 pos 28569365 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796043.

Wiscseq_DsLox233237_21C.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G54826.1-UTR3 and AT3G54830.1 (chr 3 pos 20311837 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796044.

Wiscseq_DsLox233237_21C.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G57062.2 (at chr 3 pos 21121890 (C/21121890-21121976) on the TAIR10). The sequences' 1-87 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 0, R 2, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452075.

Wiscseq_DsLox233237_21C.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G23460.1 (chr 4 pos 12248550 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796045.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox233237_21C

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G76135 AT1G76140 AT3G54826 AT3G54830 AT3G57062 AT4G23460

EMBL accession:

CW800266